This article provides an in-depth overview of Brazzein manufacturers and suppliers in Italy, emphasizing their advanced synthetic biology and fermentation production methods, as well as OEM/ODM service capabilities. It explores Brazzein's unique properties and wide-ranging applications in the food, beverage, and healthcare industries. Italy's leading companies, including Magellan Life Sciences, Brazzale S.p.A., DSM Food Specialties Italy, and Pentasweet UAB's distribution partners, are shown to be at the forefront of Brazzein innovation, offering high-quality, natural, zero-calorie sweetener solutions to meet growing global demand.